diff --git a/dev-python/jupyter-server/Manifest b/dev-python/jupyter-server/Manifest
index e7410f46e5c34..362db1b7eb20b 100644
--- a/dev-python/jupyter-server/Manifest
+++ b/dev-python/jupyter-server/Manifest
@@ -1 +1,2 @@
 DIST jupyter_server-2.15.0.tar.gz 725227 BLAKE2B da7b0106de95aa2dd3abed6d89e768a8beed1377b6a5dd0f6f9d79cd5c22cfe1259279be34771f48065ce618ae5de61ff1376a30af6c2085c481eecdec0b0d62 SHA512 96d5cf536c0ac242c86f20d0c18662f544d8d6b7a11a3fd06e473205fa597da02333f2064ffc9440ffd177c916dd37b3c608a623c35443e67663768aae97f0fc
+DIST jupyter_server-2.16.0.tar.gz 728177 BLAKE2B 4a508114057b2e54cc62e794ab8dee8934caea0bf4335928c0586190dc9cf46c9825cd006d5ae35ac82207b00e93f3edd11e45dbeaeba1baadacf472e6e6ec6b SHA512 75cce8d69a57ba5792184d16b53a75e3b83f67222ce8a3f9a7091aa0f452621d9914d639731b8497f5baf92176c405736cac6b5bf7a383e8e3523a67f738fb3c
diff --git a/dev-python/jupyter-server/jupyter-server-2.16.0.ebuild b/dev-python/jupyter-server/jupyter-server-2.16.0.ebuild
new file mode 100644
index 0000000000000..7c43a5fe6f524
--- /dev/null
+++ b/dev-python/jupyter-server/jupyter-server-2.16.0.ebuild
@@ -0,0 +1,77 @@
+# Copyright 1999-2025 Gentoo Authors
+# Distributed under the terms of the GNU General Public License v2
+
+EAPI=8
+
+DISTUTILS_USE_PEP517=hatchling
+PYTHON_COMPAT=( pypy3_11 python3_{11..13} )
+
+inherit distutils-r1 pypi
+
+DESCRIPTION="Core services, APIs, and REST endpoints to Jupyter web applications"
+HOMEPAGE="
+	https://jupyter.org/
+	https://github.com/jupyter-server/jupyter_server/
+	https://pypi.org/project/jupyter-server/
+"
+
+LICENSE="BSD"
+SLOT="0"
+KEYWORDS="~amd64 ~arm ~arm64 ~loong ~ppc ~ppc64 ~riscv ~s390 ~sparc ~x86"
+
+RDEPEND="
+	>=dev-python/anyio-3.1.0[${PYTHON_USEDEP}]
+	>=dev-python/argon2-cffi-21.1[${PYTHON_USEDEP}]
+	>=dev-python/jinja2-3.0.3[${PYTHON_USEDEP}]
+	>=dev-python/jupyter-client-7.4.4[${PYTHON_USEDEP}]
+	>=dev-python/jupyter-core-5.1.0[${PYTHON_USEDEP}]
+	>=dev-python/jupyter-server-terminals-0.4.4[${PYTHON_USEDEP}]
+	>=dev-python/jupyter-events-0.11.0[${PYTHON_USEDEP}]
+	>=dev-python/nbconvert-6.4.4[${PYTHON_USEDEP}]
+	>=dev-python/nbformat-5.3.0[${PYTHON_USEDEP}]
+	>=dev-python/overrides-5.0[${PYTHON_USEDEP}]
+	>=dev-python/packaging-22.0[${PYTHON_USEDEP}]
+	>=dev-python/prometheus-client-0.9[${PYTHON_USEDEP}]
+	>=dev-python/pyzmq-24[${PYTHON_USEDEP}]
+	>=dev-python/send2trash-1.8.2[${PYTHON_USEDEP}]
+	>=dev-python/terminado-0.8.3[${PYTHON_USEDEP}]
+	>=dev-python/tornado-6.2[${PYTHON_USEDEP}]
+	>=dev-python/traitlets-5.6.0[${PYTHON_USEDEP}]
+	>=dev-python/websocket-client-1.7[${PYTHON_USEDEP}]
+"
+BDEPEND="
+	dev-python/hatch-jupyter-builder[${PYTHON_USEDEP}]
+	test? (
+		dev-python/ipykernel[${PYTHON_USEDEP}]
+		dev-python/flaky[${PYTHON_USEDEP}]
+		dev-python/pytest-console-scripts[${PYTHON_USEDEP}]
+		>=dev-python/pytest-jupyter-0.7[${PYTHON_USEDEP}]
+		dev-python/pytest-rerunfailures[${PYTHON_USEDEP}]
+		dev-python/pytest-timeout[${PYTHON_USEDEP}]
+		dev-python/pytest-tornasync[${PYTHON_USEDEP}]
+		dev-python/requests[${PYTHON_USEDEP}]
+	)
+"
+
+distutils_enable_tests pytest
+
+python_test() {
+	local EPYTEST_DESELECT=(
+		# This fails if your terminal is zsh (and maybe other non-bash as well?)
+		tests/test_terminal.py
+		# Fails because above is ignored
+		tests/auth/test_authorizer.py
+		# Fails with additional extensions installed
+		tests/extension/test_app.py::test_stop_extension
+	)
+
+	# FIXME: tests seem to be affected by previously installed version
+	local -x PYTEST_DISABLE_PLUGIN_AUTOLOAD=1
+	epytest \
+		-p pytest_tornasync.plugin \
+		-p jupyter_server.pytest_plugin \
+		-p pytest_console_scripts \
+		-p pytest_timeout \
+		-p pytest_rerunfailures --reruns=5 \
+		-o tmp_path_retention_policy=all
+}