diff --git a/sci-biology/samtools/Manifest b/sci-biology/samtools/Manifest
index c7b62e85d9c1d..d6d0866c5ea93 100644
--- a/sci-biology/samtools/Manifest
+++ b/sci-biology/samtools/Manifest
@@ -1,6 +1,2 @@
 DIST samtools-1.20.tar.bz2 9179938 BLAKE2B b534e659899a822e191c779a6ce9247854036da3435a0b63748783edc96d610ff0f02f73bbb5c1eab3ff86dbcca331113f3312a7c3376141ef89b6a8684446e4 SHA512 8526286243d057758cb846311d0a8c728026d31438e87fcc03e0df576f33bcc6da0e18bce141dbdc438a116341c94aa92701cdf10ba6b1301eadedbb34120822
-DIST samtools-1.21.tar.bz2 9149284 BLAKE2B f4e0b155b0bc8aaea81835e751d94c121f6256340e2db3a809d1ee46bed16168a0fb43d9359bf4c3967d0b77ab1151e105107c47eb0481a2c49414ffd5f1faa2 SHA512 4f80a4333ebb4dc0eb5f38f29474424b1acca9b677aa206b111c7a638b8ae924ab2dcdc9de15eb1b849576d0158579a476a7b78ccd73e7d2baafc3bbb88c6103
-DIST samtools-1.22.1.tar.bz2 9269357 BLAKE2B 240b2166a548d398af18e5c5b1897988e4a1267988e1c3f508c990b05cfa684ca631336971d28ce08581b9d65c3b33cdb1be4cfabc5b65e210c3ae0a57215881 SHA512 31d05490f3b5d8879b7cdfe16bb628e2a1c42fdfd98873f55796b94e2d59a86cd58d7a820758d368998f0c013a26da838a7b051c01f0f22d38362ae13d069600
-DIST samtools-1.22.tar.bz2 9292743 BLAKE2B 74805efe6035d4987762c5d9c5fc1ede217f8c67e1778767c2e611c0844639149c73b7cef40649212ba3dcd11f089d0ede91f6127df31433d4e33c8f22378b93 SHA512 8bb4d68ac5f819d6e175f43d8719402f17636b958ed016a943dc6c1971704f405908562ff9fe8f3c7c8725f729057024b305a6ca428a09be8b1e63a1df1cd578
 DIST samtools-1.23.1.tar.bz2 9468164 BLAKE2B dbce77b36525498bb153381179c8978f0dd652dd2a33286817d5b093e51eebe90e7a13590306052da3cc5dd9a2262a9a6866234b02f16194ee5fc1f927ea64af SHA512 c4de14ea17786200daa50b94c41238f1241a852ee0268867d455ae5e2cbe62cd53a7891ce354ecc5a7f6b27d233812a68ce7a64206919722e586e8fe2008229a
-DIST samtools-1.23.tar.bz2 9357675 BLAKE2B 253ded3935bce0f8d7329fd1d6bafb194bf2c0821bdd378a31b0e0646f53940df252df69cc830ea4bef360285349c07cb6b13f3c1a0c8e4e1321feeb21500a84 SHA512 cf3442cd731729b5a9f9487843ea98bbb31db853c253109a97dec6e609d0df9095223ab47d8ce3cb8b3536a8d26e2f616e732d115b1340247873f41659688bac
diff --git a/sci-biology/samtools/samtools-1.21.ebuild b/sci-biology/samtools/samtools-1.21.ebuild
deleted file mode 100644
index f11e9e81198b5..0000000000000
--- a/sci-biology/samtools/samtools-1.21.ebuild
+++ /dev/null
@@ -1,47 +0,0 @@
-# Copyright 1999-2025 Gentoo Authors
-# Distributed under the terms of the GNU General Public License v2
-
-EAPI=8
-
-inherit toolchain-funcs
-
-DESCRIPTION="Utilities for analysing and manipulating the SAM/BAM alignment formats"
-HOMEPAGE="http://www.htslib.org/"
-SRC_URI="https://github.com/samtools/${PN}/releases/download/${PV}/${P}.tar.bz2"
-
-LICENSE="MIT"
-SLOT="0"
-KEYWORDS="~amd64 ~x86 ~x64-macos"
-
-RDEPEND="
-	dev-lang/perl
-	=sci-libs/htslib-$(ver_cut 1-2)*:=
-	sys-libs/ncurses:=[unicode(+)]
-	virtual/zlib:="
-DEPEND="${RDEPEND}"
-BDEPEND="virtual/pkgconfig"
-
-src_prepare() {
-	default
-
-	# remove bundled htslib
-	rm -r htslib-* || die
-}
-
-src_configure() {
-	econf \
-		--with-ncurses \
-		--with-htslib=system \
-		CURSES_LIB="$($(tc-getPKG_CONFIG) --libs ncursesw || die)"
-}
-
-src_compile() {
-	emake AR="$(tc-getAR)"
-}
-
-src_install() {
-	default
-
-	dodoc -r examples
-	docompress -x /usr/share/doc/${PF}/examples
-}
diff --git a/sci-biology/samtools/samtools-1.22.1.ebuild b/sci-biology/samtools/samtools-1.22.1.ebuild
deleted file mode 100644
index f11e9e81198b5..0000000000000
--- a/sci-biology/samtools/samtools-1.22.1.ebuild
+++ /dev/null
@@ -1,47 +0,0 @@
-# Copyright 1999-2025 Gentoo Authors
-# Distributed under the terms of the GNU General Public License v2
-
-EAPI=8
-
-inherit toolchain-funcs
-
-DESCRIPTION="Utilities for analysing and manipulating the SAM/BAM alignment formats"
-HOMEPAGE="http://www.htslib.org/"
-SRC_URI="https://github.com/samtools/${PN}/releases/download/${PV}/${P}.tar.bz2"
-
-LICENSE="MIT"
-SLOT="0"
-KEYWORDS="~amd64 ~x86 ~x64-macos"
-
-RDEPEND="
-	dev-lang/perl
-	=sci-libs/htslib-$(ver_cut 1-2)*:=
-	sys-libs/ncurses:=[unicode(+)]
-	virtual/zlib:="
-DEPEND="${RDEPEND}"
-BDEPEND="virtual/pkgconfig"
-
-src_prepare() {
-	default
-
-	# remove bundled htslib
-	rm -r htslib-* || die
-}
-
-src_configure() {
-	econf \
-		--with-ncurses \
-		--with-htslib=system \
-		CURSES_LIB="$($(tc-getPKG_CONFIG) --libs ncursesw || die)"
-}
-
-src_compile() {
-	emake AR="$(tc-getAR)"
-}
-
-src_install() {
-	default
-
-	dodoc -r examples
-	docompress -x /usr/share/doc/${PF}/examples
-}
diff --git a/sci-biology/samtools/samtools-1.22.ebuild b/sci-biology/samtools/samtools-1.22.ebuild
deleted file mode 100644
index f11e9e81198b5..0000000000000
--- a/sci-biology/samtools/samtools-1.22.ebuild
+++ /dev/null
@@ -1,47 +0,0 @@
-# Copyright 1999-2025 Gentoo Authors
-# Distributed under the terms of the GNU General Public License v2
-
-EAPI=8
-
-inherit toolchain-funcs
-
-DESCRIPTION="Utilities for analysing and manipulating the SAM/BAM alignment formats"
-HOMEPAGE="http://www.htslib.org/"
-SRC_URI="https://github.com/samtools/${PN}/releases/download/${PV}/${P}.tar.bz2"
-
-LICENSE="MIT"
-SLOT="0"
-KEYWORDS="~amd64 ~x86 ~x64-macos"
-
-RDEPEND="
-	dev-lang/perl
-	=sci-libs/htslib-$(ver_cut 1-2)*:=
-	sys-libs/ncurses:=[unicode(+)]
-	virtual/zlib:="
-DEPEND="${RDEPEND}"
-BDEPEND="virtual/pkgconfig"
-
-src_prepare() {
-	default
-
-	# remove bundled htslib
-	rm -r htslib-* || die
-}
-
-src_configure() {
-	econf \
-		--with-ncurses \
-		--with-htslib=system \
-		CURSES_LIB="$($(tc-getPKG_CONFIG) --libs ncursesw || die)"
-}
-
-src_compile() {
-	emake AR="$(tc-getAR)"
-}
-
-src_install() {
-	default
-
-	dodoc -r examples
-	docompress -x /usr/share/doc/${PF}/examples
-}
diff --git a/sci-biology/samtools/samtools-1.23.ebuild b/sci-biology/samtools/samtools-1.23.ebuild
deleted file mode 100644
index 1c202445e9de6..0000000000000
--- a/sci-biology/samtools/samtools-1.23.ebuild
+++ /dev/null
@@ -1,47 +0,0 @@
-# Copyright 1999-2026 Gentoo Authors
-# Distributed under the terms of the GNU General Public License v2
-
-EAPI=8
-
-inherit toolchain-funcs
-
-DESCRIPTION="Utilities for analysing and manipulating the SAM/BAM alignment formats"
-HOMEPAGE="http://www.htslib.org/"
-SRC_URI="https://github.com/samtools/${PN}/releases/download/${PV}/${P}.tar.bz2"
-
-LICENSE="MIT"
-SLOT="0"
-KEYWORDS="~amd64 ~x86 ~x64-macos"
-
-RDEPEND="
-	dev-lang/perl
-	=sci-libs/htslib-$(ver_cut 1-2)*:=
-	sys-libs/ncurses:=[unicode(+)]
-	virtual/zlib:="
-DEPEND="${RDEPEND}"
-BDEPEND="virtual/pkgconfig"
-
-src_prepare() {
-	default
-
-	# remove bundled htslib
-	rm -r htslib-* || die
-}
-
-src_configure() {
-	econf \
-		--with-ncurses \
-		--with-htslib=system \
-		CURSES_LIB="$($(tc-getPKG_CONFIG) --libs ncursesw || die)"
-}
-
-src_compile() {
-	emake AR="$(tc-getAR)"
-}
-
-src_install() {
-	default
-
-	dodoc -r examples
-	docompress -x /usr/share/doc/${PF}/examples
-}