diff --git a/sci-libs/htslib/Manifest b/sci-libs/htslib/Manifest
index fce2951cfeb6a..11149f73b75a6 100644
--- a/sci-libs/htslib/Manifest
+++ b/sci-libs/htslib/Manifest
@@ -1,2 +1 @@
-DIST htslib-1.20.tar.bz2 4779028 BLAKE2B def7a135f37a4c7a167ada554b92b0e0107ab3dcbdab69abdea387035159f40fb183477275c137b0d786fdfc7264303898f98c54acb1714f9b7cb21e22612143 SHA512 a156f5c895fdae77f976f463a2d4d1111dc056893ef45b7e9cf62e9115898bc8cb47465bb1779e534c26943df478892385d44646e97bd0fa67fceb83ef7a649b
 DIST htslib-1.23.1.tar.bz2 4987046 BLAKE2B bb8beb6ce435bb8a940c70edd830c54c90a626de553ddfb159a68cd9fca97a608ee2a4b3ae820b52730bf3be2fd0b8e1b1776f005bf18a39c6237df0451448a8 SHA512 abe3c7cf37c2300b5d2b82f9f0a88cb1b29f289aad829d2c1c7e39ca2ddafd9fd43efea3301a7e1fd1be1b91e5fbc32511961bb20f2aa1a58729ac84603d59f0
diff --git a/sci-libs/htslib/htslib-1.20.ebuild b/sci-libs/htslib/htslib-1.20.ebuild
deleted file mode 100644
index 43627c7821afa..0000000000000
--- a/sci-libs/htslib/htslib-1.20.ebuild
+++ /dev/null
@@ -1,52 +0,0 @@
-# Copyright 1999-2025 Gentoo Authors
-# Distributed under the terms of the GNU General Public License v2
-
-EAPI=8
-
-inherit toolchain-funcs
-
-DESCRIPTION="C library for high-throughput sequencing data formats"
-HOMEPAGE="http://www.htslib.org/"
-SRC_URI="https://github.com/samtools/${PN}/releases/download/${PV}/${P}.tar.bz2"
-
-LICENSE="MIT"
-SLOT="0/3"
-KEYWORDS="amd64 ~x86"
-IUSE="+bzip2 curl +lzma"
-
-RDEPEND="
-	virtual/zlib:=
-	bzip2? ( app-arch/bzip2 )
-	curl? ( net-misc/curl )
-	lzma? ( app-arch/xz-utils )"
-DEPEND="${RDEPEND}"
-
-src_prepare() {
-	default
-
-	# upstream injects LDFLAGS into the .pc file,
-	# which is a big nono for QA
-	sed -e '/^\(static_l\|Libs.private\|Requires.private\)/d' \
-		-i htslib.pc.in || die
-}
-
-src_configure() {
-	econf \
-		--disable-gcs \
-		--disable-plugins \
-		--disable-s3 \
-		$(use_enable bzip2 bz2) \
-		$(use_enable curl libcurl) \
-		$(use_enable lzma)
-}
-
-src_compile() {
-	emake AR="$(tc-getAR)"
-}
-
-src_install() {
-	default
-
-	# doesn't use libtool, can't disable static libraries
-	find "${ED}" -name '*.a' -delete || die
-}